After many delays (mea culpa!), we finally posted our work on P2X2 receptor gating on bioRxiv. Huge thanks to Federica Gasparri, first-ever PhD student of the Pless lab, who was later joined by Sarune Bielickaite and Mette H. Poulsen on the project. We show that mutating the inter-subunit interfaces consistently increases apparent ATP affinity.