Proud to present our first foray into protein semi-synthesis. Using Nav1.5, P2X2R and GFP as examples, we show that tandem protein trans-splicing can used to insert synthetic peptides into proteins in live cells. The project was lead by two fantastic postdocs, Keith Khoo and Iacopo Galleano, with assistance from a number of other lab members, as well as our collaborators from the Tampé group in Frankfurt. Full paper can be found on bioRxiv.
